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Sbl 202: Laboratory Techniques Question Paper

Sbl 202: Laboratory Techniques

Course:Bachelor Of Science In Microbiology And Biotechnology

Institution: University Of Nairobi question papers

Exam Year:2010

UNIVERSITY OF NAIROBI
SECOND SEMESTER EXAMINATIONS 2012/2013
SECOND YEAR EXAMINATIONS FOR THE DEGREE OF BACHELOR OF SCIENCE
SBL 202: LABORATORY TECHNIQUES
DATE: JULY 23, 2010 TIME: 2.00 PM – 5.00 PM
INSTRUCTIONS
Answer ANY 10 questions. Each is 7 marks. Illustrate your answers with well- labeled diagrams where appropriate.
1. Distinguish between ED50 and LD50 and list three factors modifying the response of animal to drugs.
2. Describe how you would rear a colony of Aedes aegypti in the laboratory.
3. Write a concise description of thin layer chromatography technique.
4. Describe how you would use centrifugation to isolate sub- cellular fractions.
5. Give an outline of the principles and applications of electrophoresis technique.
6. Outline the principles and applications of affinity chromatography.
7. List 14 safety precautions in the laboratory.
8. Describe the immunofluorescence technique and list its applications.
9. Give a classification of various soil target taxa and elucidate their importance in soil fertility analysis.
10. Outline the methods of ecological sample collection.
11. Distinguish between the following:

a. Absorption and emission spectra
b. Line and band spectra
12. Give an account of the role of spectrophotometry in enzyme assays.
13. Describe the ELISA technique its principles and applications.
14. Describe the half life of an isotope and list four applications of radio isotope techniques in biology.
15. List 7 routes of drug administration to laboratory animals.

Sbl 202: Laboratory Techniques Question Paper

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