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Sbl 202: Laboratory Techniques Question Paper

Sbl 202: Laboratory Techniques

Course:Bachelor Of Science In Microbiology And Biotechnology

Institution: University Of Nairobi question papers

Exam Year:2013

UNIVERSITY OF NAIROBI
SECOND SEMESTER EXAMINATIONS 2012/2013
SECOND YEAR EXAMINATIONS FOR THE DEGREE OF BACHELOR OF SCIENCE
SBL 202: LABORATORY TECHNIQUES
DATE: MAY 15, 2013 TIME: 9.00 AM – 12.00 AM
INSTRUCTIONS
Answer ANY 10 questions. Each is 7 marks. Illustrate your answers with well- labeled diagrams where appropriate.
1. List seven examples of laboratory animal welfare practices.
2. Explain the process and utility of mark recapture technique as used in ecological studies.
3. Describe how you would rear a colony of Aedes aegypti in the laboratory.
4. Explain how you would separate a mixture of amino acids using thin layer chromatography.
5. Describe how you would establish a culture of mammalian cells in vitro.
6. Outline the principles and applications of the gel filtration technique.
7. Describe the gas liquid chromatography technique and list its applications.
8. Give the concise description of two techniques used to measure pH.
9. Compare the advantages and disadvantages of remote sensing with conventional in situ environmental monitoring.
10. Explain the criteria for selection of buffers for biological studies.
11. (a). List three techniques used to detect radioactivity.
(b). List four applications of radioisotopes in Biology.
12. Describe a named biological assay.
13. Illustrate the separation of intracellular organelles using differential centrifugation.
14. Give an outline of the principles and applications of the electrophoresis techniques.
15. Describe the ion exchange chromatography technique.

Sbl 202: Laboratory Techniques Question Paper

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