Bio425: Biotechnology Development Question Paper
Bio425: Biotechnology Development
Course:Agriculture And Natural Resources
Institution: Kenya Methodist University question papers
Exam Year:2011
Instructions:
Answer SECTION A (Compulsory) and any two questions in SECTIOIN B.
Question One.
Define the following terms:
(10mks)
DNA
Chimera
Cosmid
Lambda phage
Vehicle
Differentiate between plasmid vectors and lambda vectors.
(10mks)
Explain the need for optimization of polymerase chain reaction conditions
(10mks)
SECTION B – 40 MARKS
Question Two
Explain the use of bacteriophage lambda as a vector rather than a plasmid (20mks)
Question Three.
Discuss the methods in DNA transfection. (20mks)
Question Four
Discuss the factors that influence the in vitro approach to germplasm conservation. (20mks)
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