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Bio425: Biotechnology Development Question Paper

Bio425: Biotechnology Development

Course:Agriculture And Natural Resources

Institution: Kenya Methodist University question papers

Exam Year:2011

Instructions:

Answer SECTION A (Compulsory) and any two questions in SECTIOIN B.

Question One.

Define the following terms:

(10mks)

DNA
Chimera
Cosmid
Lambda phage
Vehicle

Differentiate between plasmid vectors and lambda vectors.

(10mks)

Explain the need for optimization of polymerase chain reaction conditions

(10mks)

SECTION B – 40 MARKS

Question Two

Explain the use of bacteriophage lambda as a vector rather than a plasmid (20mks)

Question Three.
Discuss the methods in DNA transfection. (20mks)

Question Four
Discuss the factors that influence the in vitro approach to germplasm conservation. (20mks)

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