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04-A:Histology Question Paper

04-A:Histology

Course:Diploma In Medical Laboratory Sciences

Institution: Kenya Medical Training College question papers

Exam Year:2008

KENYA MEDICAL TRAINING COLLEGE KITUI

DEPARTMENT OF MEDICAL LABORATORY SCIENCES

P.O BOX 711

KITUI

SEPTEMBER 2008 MLS CLASS END OF 2ND YEAR EXAM

HISTOLOGY PAPER

DATE 16/12/2008

TIME 2 HOURS

8:30AM-10:30PM

INSTRUCTIONS TO THE CANDIDATE
1.Write your college number on all sheets of paper used.
2.Do not write your name on the sheets of paper used.
3.Attempt all questions in section I and II.
4.Cycle the correct response(s) in section I.
5.For any wrong response you will be deducted half a mark.
6.The paper takes 2 hours.

SECTION I (M.C.Q 40 MARKS)

1.The following are cytoplasmic inclusions except?
a)mucin
b)vacuoles
c)golgi apparatus
d)yolk
e)centrioles

2.Simple columnar epithelial cells are found in the:
a)alimentary canal
b)lungs
c)gall bladder
d)brain
e)retina of the eye

3.The following features are found in the spleen:
a)red pulp
b)white pulp
c)kupffer cells
d)goblet cells
e)paneth cells

4.Characteristics of glycogen within a cell undergoing autolysis are?
a)diminishes
b)karyolysis
c)pyknosis
d)empty spaces
e)become granular

5.Which one of the following is criteria of a good fixative?
a)must fortify the tissue against harsh effects of solutions to be used later
b)must render tissue substances soluble
c)must activate enzymes
d)must have negative effect on staining
e)must penetrate quickly and easily

6.The following are associated with osimic acid:
a)preserve cytoplasm
b)used in dark ground microscopy
c)causes conjuctivitis
d)storage should be in fume cupboard
e)penetrate quickly and evenly

7.The following is/are surface softener(s):
a)formaldehyde
b)perenyl fluid
c)orths fluid
d)muller fluid
e)clarkes fluid

8.Nucleus are present in all types of cells except:
a)white blood cells
b)platelets
c)red blood cells
d)lymphocytes
e)basophils

9.Post-chroming helps to demonstrate:
a)mitochondria and myelin
b)RNA and DNA
c)lysosome and golgi apparatus
d)centrioles and mitochondria
e)mucin and DNA

10.Polysterine resin used in ion exchange include:
a)zeokarb 250
b)formic acid
c)zeokarb 225
d)nitric acid
e)urea

11.The following are characteristics of a good de-calcifying solution except:
a)should damage the tissue
b)reasonable speed of de-calcification
c)should completely remove calcium
d)impairment of subsequent staining technique
e)should not damage the tissue

12.Dioxane......................
a)causes shrinkage
b)is miscible with water
c)is used routinely
d)is miscible with water
e)has got some characteristics with ethanol

13.Synthetic resins are mainly used for:
a)soft specimen
b)de-calcified bones
c)unde-calcified bones
d)for sections to be examined using electron microscope
e)for tissues with differing consistency

14.The following are found in polarized microscope:
a)exciter filter
b)barrier filter
c)polarizer
d)analyzer
e)UV light

15.The difference between phase contrast microscopy and interference microscopy is:
a)phase contrast microscopy exaggerates minute differences in refractive index whereas interference does not
b)phase contrast microscopy utilizes short wavelength unlike interference microscopy
c)phase contrast utilizes dark illumination unlike interference
d)phase contrast produces varities of brightness unlike interference
e)interference generates its own light rays whereas phase contrast does not

16.Vacuum impregnation is exceptionally good for the following tissue:
a)heart
b)fetus
c)lung
d)breast
e)intestine

17.Which one of the following will cause the tissue to float on the surface?
a)toulene
b)chloroform
c)xylene
d)benzene
e)carbon tetra chloride

18.Cryostat microtome is good for
a)frozen section
b)liver
c)brain
d)fatty tissue
e)hard tissues

19.The following are used in plate
a)hanging
b)flexible
c)medium abrasive powder
d)fine abrasive powder
e)carborudum

20.Starch paste is not used in demonstration of
a)carbohydrates
b)lipids
c)proteins
d)vitamins
e)nucleus

21.Litmus dye is obtained from:
a)saffron
b)lichen
c)coccus cacti
d)croccus sative
e)rocella tinctoria

22.Polysterine in DPX is:
a)disterene
b)butylpthalate
c) xylene
d)styrene
e)xam

23.The following are angetaffin cells:
a)brain
b)eye
c)melanin
d)adrenal gland
e)phenolic compound found in kultschitzky cells

24.Accentuators include:
a)phenol
b)picric acid
c)carbol fuschin
d)Orange G
e)carmic acid

25.Examples of mordants are:
a)toluidine blue
b)copper
c)thiazine
d)iron
e)thionine

SECTION II (60 MARKS)
1.Diagrammatically describe how flourescence microscopy functions (20 marks)

2.a)Describe physical theory of staining (10 marks)
b)Briefly explain how inorganic haemosiderin in ferric state is demonstrated (10 marks)

3.Explain the following terminologies (20 marks)
a)corpora amylacea
b)accentuators
c)elective solubility
d)causes of section detachment from slide
e)scores

04-A:Histology Question Paper

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