Mbio 205:Advanced Microbiology Question Paper
Mbio 205:Advanced Microbiology
Course:Medical Laboratory
Institution: Kenya Methodist University question papers
Exam Year:2013
KENYA METHODIST UNIVERSITY
END OF 1''ST ''TRIMESTER 2013 (EVENING) EXAMINATION
SCHOOL : MEDICINE & HEALTH SCIENCES
DEPARTMENT : MEDICAL LABORATORY SCIENCE
UNIT CODE : MBIO 205
UNIT TITLE : ADVANCED MICROBIOLOGY
TIME : 3HOURS
INSTRUCTIONS: Answer ALL Questions
SECTION A
Question One
Match the following growth characteristics with their respective temperature
ranges A to C.
Psycholopiles
A. Grows between 55-66oc
Mesophiles
B. Grow well between 25 -45oc
Thermophiles
C. Grow below 25oc
Question Two
Streptomycin is obtained from
Streptococcus species
Streptomyces griseus
Staphylococcus aureus
None of these.
Question Three
?-lactum ring is present in
Erythromycin
Penicillin
Tetanolysin
Chloramptienical
Question Four
Toxin produced by C-botulinum is
Botulin
Tetracylin
Tetanolysin
Cholaragen.
Question Five
Enythromycin inhibits protein synthesis by
Attaching to 305 ribosome unit
Attaching to 505 unit on ribosome
By attachment of t-RNA
By the attachment of m-RNA.
Question Six
The condition required for autoclave
121oc temp and 15 lbs, pressure for 20 min
120oc temp and 20lbs pressure for 30 min
150oc temp for 1 hour
130oc temp for 2 hours
Question Seven
The significance of the plasma membrane is that
It selectively allows some molecules to pass into the organism
It prevents movement of molecules out of the organism
It is the site of protein synthesis
All of the above.
Question Eight
What is a plasmid?
Self replicating segment of double stranded DNA
Self replicating segment of single stranded RNA
A bacterial chromosome
None of the above.
Question Nine
Gram positive cells
Have a second, outer membrane that helps retain the crystal violet stain.
Have multiple layers of petidoglycan that help retain the crystal violet stain
Have a thick capsule that traps the crystal violet stain
Have a periplasmic space that traps the crystal violet.
Question Ten
Fimbriae
Attach bacteria to various surfaces
Cause bacteria mover through fluids
Sense changes in nutrient concentration
Are pathways for the secretion of exoenzymes.
Question Eleven
The term obligate anaerobe refers to an organism that
Does not use oxygen but tolerates it
Is killed by oxygen
Uses oxygen when present or grows without oxygen even when oxygen is present
Requires less oxygen than is present in air.
Question Twelve
A mutagen is
A chemical or physical agent that induces mutations.
An enzyme that repairs mutations
A molecule that stabilizes DNA thus prevents mutations from occurring
All of the above.
Question Thirteen
Griffith (1928) reported the phenomenon of transformation first in
H. influenza
Bacillus SPP
Pneumococci
E.coli
Question Fourteen
The lay phase in the growth curve of bacteria shows that
The cells are dormant
The cells are active in the synttess of cell components, but not dividing
The cells are starting the cell division, but the division is not complete
The cells take rest.
Question Fifteen
Louis Pasteur is NOT concerned with the discovery of
Bacterial attenuation
Sterilization
Growth requirements of microbes
Staining technique of bacteria
Question Sixteen
Mode of action of quinolore antibiotics on growth bacteria is thought to be
Inhibition of a lactamase
Prevention of the cross linking of glycine
Inhibition of DNA gyrase
Inhibition of reverse transcriptase
Question Seventeen
Match the following enzymes with their activities A to E
Hyalurunidase
A. inactivates leucocytes and acid bacterial
Invasion
Collagenase
B. Reversibly catalyzes the breakdown of
major component
Lecithinase
C. Disintegrates a constituent of muscle
cartilage and bone.
Leucocidins
D. haemolysis of enythrocytes.
SECTION B
Question Eighteen
Draw a well labeled diagram of bacteria. (5marks)
Question Nineteen
Describe the structures external to the cell membrane of prokaryotic cells.
(5marks)
Question Twenty
Describe water availability as a factor in microbial growth. (5marks)
Question Twenty one
Discuss inhibition of cell wall synthesis by antimicrobial agents. (5marks)
SECTION C
Question Twenty two
Describe the normal flora bacteria of the skin, respiratory tract and intestinal tract of human. (15marks)
Question Twenty three
Explain invasion, inflammation an extracellular survival mechanisms as a
stage in bacterial pathogenesis. (15marks)
Question Twenty four
Discuss any THREE opportunistic mycoses that include disease in immune compromised persons. (15marks)
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