Mlsc 204: Cellular And Molecular Biology Question Paper
Mlsc 204: Cellular And Molecular Biology
Course:Medical Laboratory Sciences
Institution: Kenya Methodist University question papers
Exam Year:2012
KENYA METHODIST UNIVERSITY
END OF 2''ND ''TRIMESTER 2012(EVENING) EXAMINATIONS
SCHOOL : MEDICINE & HEALTH SCIENCES
DEPARTMENT : MEDICAL LABORATORY SCIENCES
UNIT CODE : MLSC 204
UNIT TITLE : CELLULAR AND MOLECULAR BIOLOGY
TIME : 3HOURS
INSTRUCTIONS: Answer ALL Questions in Section A and TWO in Section B
SECTION A
QUESTION ONE
Briefly describe the central dogma of molecular biology. (3marks)
QUESTION TWO
Differentiate between the virus and the plasmid. (2marks)
QUESTION THREE
State the THREE functions of homologous recombination within and among DNA molecules in a cell. (3marks)
QUESTION FOUR
State THREE steps involved in processing of pre-mRNA. (3marks)
QUESTION FIVE
Distinguish the following terms: promoter and operator. (2marks)
QUESTION SIX
Discuss the THREE phases in interphase of mitosis. (3marks)
QUESTION SEVEN
State FOUR factors that influence allele frequency in a population. (4marks)
QUESTION EIGHT
Describe the FIVE stages of transcription. (10marks)
SECTION B
QUESTION ONE
a) Discuss SIX clinical applications of PCR. (12marks)
b) Discuss the principles of PCR. (8marks)
QUESTION TWO
With the aid of a diagram explain the processes of mitosis and meiosis for a diploid cell with n=2, 2n=4 chromosomes. What are the similarities and differences between these two modes of cell division and what role does mitosis and meiosis play in human reproduction and development? (20marks)
QUESTION THREE
a) What is the function of the following enzymes or proteins in DNA replication, primase ligase, DNA polymerase 5-3 pollymerizing activity, helicase, single stranded DNA topoismerase? Describe what would happen during DNA replication mutation occurred in the genes encoding each of these proteins. (6marks)
b) Describe a replication fork and label the 5’ and 3’ ends. Include the leading and lagging strands in your drawing. Beneath the drawing indicate the direction the replication fork is moving. Why is the 3 OH group on the deoxyribose ring so important for DNA synthesis? (8marks)
c) Discuss THREE mechanisms of DNA repair during replication. (6marks)
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