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Dna Technology Question Paper

Dna Technology

Course:Bachelor Of Science In Biochemistry

Institution: Egerton University question papers

Exam Year:2007

EGERTON UNIVERSITY
UNIVERSITY EXAMINATIONS
NJORO CAMPUS
FIRST SEMESTER 2006/2007 ACADEMIC YEAR
DEGREE FOR BACHELOR OF SCIENCE
BIOC 409: DNA TECHNOLOGY

Instructions: ATTEMPT ALL QUESTIONS

Section A (40 Marks)

1. (a) What is the basic structure of a nucleoside?
Write the structure of a ribonucleoside containing 5-methyl cytosine. [2 marks]

(b) What are the functions of nucleotides in the cell? [5 marks]

2. (a) How is pulsefield electrophoresis used to resolve very long DNA molecules? [2 marks]

(b) Why does supercoiled DNA migrate faster through a gel matrix compared to relaxed circular DNA or linear DNA? [2 marks]

(c) In electrophoretic separation, the rate of migration of linear ds DNA is proportional to its molecular weight. This is not so for the RNA molecule. Why?

3. (a) Describe three commonly used non-radioactive gel detection methods. [3 marks]

(b) Using appropriate diagrams, distinguish the following two methods of labeling DNA:
"Labeling by incorporation" and "end labeling". [4 marks]

(c) How is plant steroid digoxygenin used to label DNA? [4 marks]

4. What is the difference between southern and northern blot analysis? [4 marks]

5. Describe how micro array assays are used to carry out genome wide analysis of gene expression profiles. [5 marks]

6. How are sythentic oligonucleotides used to carry out the prenatal diagnosis of sickle cell anemia? [5 marks]

Section B (30 Marks) ANSWER ALL QUESTIONS

7. (a) Vector DNAs widely used in gene cloning typically have three common characteristics. Discuss these characteristics. [6 marks]

(b) What is an expression Vector? [1 mark]

8. (a) Explain how 2', 3' dideoxynucleoside triphosphates (dd NTPs) are used to elucidate the sequence of a DNA fragment in the chain termination sequencing method . [8 marks]

(b) Briefly describe the shortgun sequencing method. [4 marks]

9. (a) Explain how the immunoaffinity chromatographic technique is used to purify individual proteins. [3mark]

(b) How does the above method differ from ion exchange chromatography? [2 marks]

(c) How can "predicted" protein coding genes be validated experimentally? [3 marks]

Dna Technology Question Paper

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