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Integrated Laboratory Techniques Ii Question Paper

Integrated Laboratory Techniques Ii

Course:Bachelor Of Science In Biochemistry

Institution: Egerton University question papers

Exam Year:2007

UNIVERSITY EXAMINATIONS

FIRST SEMESTER 2006/2007

YEAR FOUR EXAMINATIONS FOR THE DEGREE OF BACHELOR OF SCIENCE

INTERGRATED LABORATORY TECHNIQUES 111

TIME:2 HRS

INSTRUCTIONS:

Attempt ALL questions.

1.(a) What are the roles of the following reagents in DNA isolation?

(i) Proteinase K

(ii) Phenol

(iii) Lysozyme

(iv) Sdium Dodecyl Sulphate

(b) What is the principle of photometric determination of DNA concentration and purity? (10 marks)

2.Discuss the albumin major cross match of blood under the following subheadings:

(a) Purpose of the test

(b) Scientific basis of the test

(c) Detailed procedure for performing the test

(d) Expected results and their interpretation (15 marks)

3.(i) Describe the principle of operation of Warburg manometer.

(ii) State the advantage of manometry over gas-sensing electrode.(10 marks)

4.Given the following peptide,

Thr-Asn-Val-Lys-Ala-Trp-Gly-Lys (10 marks)

Explain with illustrations how the amino acid sequence of the peptide can be determined.

5.(a) What is the purpose of doing vitamin analysis.

(b) List the methods used in vitamin analysis.

(c) Briefly describe flame absorption sepctrophotometry.(15 marks)

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