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Recombination Dna Technique Question Paper

Recombination Dna Technique

Course:Bachelor Of Science In Biochemistry

Institution: Egerton University question papers

Exam Year:2007

FIRST SEMESTER 2006/2007

YEAR FOUR EXAMINATIONS FOR THE DEGREE OF BACHELOR OF SCIENCE IN BIOMEDICAL SCIENCE AND TECHNOLOGY

BMED 420-RECOMBINATION DNA TECHNIQUES

TIME:2 HRS

INSTRUCTIONS:

SECTION A: Attempt all Questions (30 marks)

1.Define the following terms as applied in recombination DNA technology.

(a) Recombinant DNA (2 marks)

(b) Transformation (2 marks)

(c) Southern blotting (2 marks)

(d) Cloning vector (2 marks)

2.Show specific reactions where the following DNA manipulative enzymes are applied in genetic engineering experiments.

(a) Klenow Fragment

(b) Terminal Nucleotidyl Tranferase

(c) DNA ligase

(d) Polynucleotidyl Kinase

3.(a) Distinguish between genomic and cDNA libraries.(4 marks)

(b) Distinguish between screening and selection of recombinants(4 marks)

(c) List the desirable characteristics of a cloning vector and explain why.(6 marks)

SECTION B:Attempt all Questions (40 marks)

4.Explain why the gel electrophoresis of DNA is essential to recombinant DNA technology.(8 marks)

5.Explain some of the challenges that complicate the expression of a eukaryotic gene that encodes a specific protein using a bacterial expression vector.How can this problem be circumvented?(10 marks)

6.Illustrate the process of screening for recombinant cells using nucleic acid probes.(10 marks)

7.Discuss the application of recombinant DNA technology in Health and forensic sciences.(12 marks)

Recombination Dna Technique Question Paper

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