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Integrated Laboratory Techniques I Question Paper

Integrated Laboratory Techniques I

Course:Bachelor Of Science In Biochemistry

Institution: Egerton University question papers

Exam Year:2005

SECOND SEMESTER 2004/2005

SECOND YEAR EXAMINATION FOR THE DEGREE OF BACHELOR OF SCIENCE

BIOC 203: INTEGRATED LABORATORY TECHNIQUES I

TIME:2 HRS

INSTRUCTIONS:

Attempt Question one & any other two.

SECTION A: Answer any 3 Questions.

1.(a) Differentiate a cation exchange resin from an anion exchange resin.

(b) A solution containing glycine,glutamic acid and lysine amino acids at pH 6.5 was loaded onto a column of carboxyl methyl cellulose. The column was eluted with a pH 6.5 buffer,with an increasing gradient of pH. Predict with explanation the elution pattern.(17 ½ marks)

2.Asprin,Caffeine,acetaminophen and phenacetin are compounds found in analgesic tablets. Explain how you would identify the compounds found in a new analgesic product for Asprin,Caffeine and acetaminophen using High performance liquid chromatography (HPLC).(17 ½ marks)

3.Your job after college is with a food industry in Nairobi.You will be required to choose a quick and inexpensive laboratory technique for fatty acids identification in various brands of cooking oil.

(a) Which laboratory technique are you going to use for the analysis? Why?

(b) Give the principles involved in (a) above.(17 ½ marks)

4.Explain the principles involved in the separation of protein by salt precipitation technique. Use serum protein in your explanation.(17 ½ marks)

SECTION B

Answer all questions

5.Why is gas-solid chromatography not used,nearly as extensively as gas-liquid chromatography in biochemical investigations?(6 marks)

6.Explain with specific examples the nature of each of the following chromatographic methods is most applicable

(a) gel-permeation

(b) Affinity.(6 marks)

7.(a) Calculate the Hydrogen ion concentration of Blood plasma, pH 7.42.(3 marks)

(c) Given the molar absorbance coefficient of tyrosine in water as 1420l mol-1 cm-1 at 275 nm.What is the concentration of tyrosine in a solution?(2 ½ marks)

Integrated Laboratory Techniques I Question Paper

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