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Sbt 415 Molecular Biology Ii Question Paper

Sbt 415 Molecular Biology Ii

Course:Microbiology And Biotechnology

Institution: University Of Nairobi question papers

Exam Year:2007

1
SUPPLEMENTARY EXAMINATIONS 2007/2008
SBT 415 MOLECULAR BIOLOGY II
DATE: ……………………... TIME:……………….
…………………………………………………………………………………………..
Answer ALL questions from SECTION A and TWO selected from SECTION
SECTION A: SHORT ANSWER QUESTIONS (50 Minutes)
Use the space provided in the question paper. (3 marks each)
1. State three factors that determine the number of amplified fragments generated by PCR during a RAPD assay (6marks )
2. Give three reasons for the incompatibility of eukaryotic genes in prokaryotic cells (10marks )
3. You have cloned a gene from the Petunia and want to know which tissue - leaves, stems, roots, flowers, or seeds, transcribes this gene to the greatest extent. How would you use a Northern blot to answer this question? (10marks )
4. Explain the drawback in the use of linkers in modifying DNA ends so that they can have sticky ends? (10marks)
5. Outline the steps involved in the use of PCR to screen for human gene mutations that might lead to genetic diseases. (6 marks)
6. How is the insulin polypeptide recovered from insulin-ß-galactosidase fusion protein?
(8 marks)
7. State three possible explanations on how antisense RNA functions. (6 marks)
8. Describe the radiolabelling of DNA by primer extension (12 marks)
9. Outline the steps involved in the use of ELISA to test for Herpesvirus. (8marks )
10. (a) What is a reading frame and when is it said to be open? (2 marks )
(b) What is a fusion protein? (2 marks)
2
SECTION B: ESSAY QUESTIONS (70 MINUTES).
Write an essay on any TWO of the following questions. Each essay is worth 20 marks. (Use the examination answer book provided).
1 Explain how Southern blot hybridization is performed to detect minute amounts of DNA.
2 Explain the molecular basis of restriction fragment length polymorphism (RFLP).
3 Discuss the production of recombinant somatotropin in E. coli.
4 How could cotton plants be genetically modified so as to acquire protection from insect larvae through expression of Bacillus thuringiensis toxin gene?

Sbt 415 Molecular Biology Ii Question Paper

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