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Sbt 415 Molecular Biology Ii Question Paper

Sbt 415 Molecular Biology Ii

Course:Microbiology And Biotechnology

Institution: University Of Nairobi question papers

Exam Year:2006

1
FIRST SEMESTER EXAMINATIONS 2006/2007
FOURTH YEAR EXAMINATIONS FOR THE DEGREE OF BACHELOR OF
SCIENCE
SBT 415 MOLECULAR BIOLOGY II
DATE: ……………………... TIME:……………….
…………………………………………………………………………………………..
Answer ALL questions from SECTION A and TWO selected from SECTION B
SECTION A: SHORT ANSWER QUESTIONS (50 Minutes) 3 marks each question
Use the space provided in the question paper.
1. Distinguish between a nick and a discontinuity in DNA and name the enzymes that repair them. (3 marks)
2. Outline the steps involved in the use horseradish peroxidase in detection of DNA of interest in an agarose gel.
3. State six applications of PCR in clinical diagnosis
4. Outline the steps involved in the use of (enzyme-linked immunosorbent assay (ELISA) to test for Herpesvirus.
5. Describe briefly orthogonal field alternation gel electrophoresis.
6. Write down the steps involved in the production of plasminogen activator in the milk of transgenic sheep.
7. Compare restriction fragment length polymorphisms (RFLPs) and randomly amplified polymorphic DNAs (RAPDs) in the aspects given in the table below
Aspect being compared
RFLP
RAPD
Quality of DNA required
Use of radioisotopes?
Detectable in all tissues?
8. Explain briefly the general principles of DNA sequencing.
9. Complete the following table with inducer and repressor of the given promoters.
Name of promoter
Repressor
Inducer
Trp promoter
Glucoamylase promoter
Human heat-shock promoter
10. Explain briefly how recombinant vaccine against hepatitis B virus (HBV) is produced in yeast. (10 lines)

SECTION B: ESSAY QUESTIONS (70 MINUTES).
Write an essay on any TWO of the following questions. Each essay is worth 20 marks. (Use the examination answer book provided).
1 Explain the mechanism of action of antisense RNA and describe how it is used to produce transgenic tomato fruits that have a long shelf life.
2 Explain why eukaryotic genes are incompatible in prokaryotic cells and how such incompatibility is avoided.
3 Discuss how crop plants can be protected from the herbicide glyphosate
4 Explain how Southern blot hybridization is performed to detect minute amounts of DNA.

Sbt 415 Molecular Biology Ii Question Paper

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