Sbt2102:General Microbiology Question Paper
Sbt2102:General Microbiology
Course:Bachelor Of Horticulture
Institution: Meru University Of Science And Technology question papers
Exam Year:2014
QUESTION ONE – (30 MARKS)
(a) Define the following terms as applied in microbiology. (6 Marks) (i) Surfactants (ii) Peptidoglycan (iii) Mesophiles (iv) Thermal Death point (v) Chemoautotrophs (vi) Bioremediation
(b) Virus structures come in different preservations depending on the arrangement of the protein layer and genetic information. Describe. (8 Marks) (c) Discuss flagellation in bacteria. (5 Marks) (d) Draw a schematic presentation of bacteria cell wall showing the differences between gram negative and gram positive bacteria. (6 Marks) (e) Outline five features unique to fungi in relation to other organisms. (5 Marks)
QUESTION TWO – (20 MARKS)
(a) Discuss reproduction in fungi. (10 Marks) (b) Describe the basic steps involved in replication of animal viruses. (10 Marks)
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QUESTION THREE – (20 MARKS)
You are given a mixed bacterial culture from which you are required to isolate a single pure culture. Discuss five methods that you can use in the laboratory to isolate and identify bacteria. (20 Marks)
QUESTION FOUR – (20 MARKS)
(a) A flesh eating streptococcus pyogenes divides every 10 minutes at body temperature. You fall down and get a knee injury and ge infected with 5 S. Pyogenes cells. (i) After 2 hours without medication, how many bacterial cells will be ravaging your body. (5 Marks) (ii) Assuming that for every ten thousand bacteria cells, a centimeter of flesh is consumed, how much tissue would have been lost after 2 hours. (5 Marks) NB: “The problem is not factual” (b) Outline the Koch Postulates which help to identify the etiological cause of disease and state cases where they do not apply. (10 Marks)
QUESTION FIVE – (20 MARKS)
(a) Discuss the general considerations for sterilization and disinfection. (15 Marks) (b) Describe the most common ingredients for culture media. (5 Marks)
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