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Sbt2255:Botanical Techniquies Question Paper

Sbt2255:Botanical Techniquies 

Course:Bachelor Of Crop Protection

Institution: Meru University Of Science And Technology question papers

Exam Year:2012



QUESTION ONE (30 MARKS)
a) List the three main rooms’ essential in a plant tissue culture lab. (3 Marks) b) Explain the following terms i. P.C.R ii. Sequencing (DNA) iii. Cloning iv. Ligation v. Restriction enzymes (10 Marks) c) Outline the gram staining process. (6 Marks) d) Distinguish between the three main blotting techniques. (3 Marks) e) Explain the main steps of preparing a herbarium specimen. (4 Marks) f) Illustrate the various stages/cycles taken by a thermocylcer in amplification process. (4 Marks)
QUESTION TWO (15 MARKS)
Outline and explain the process of separating the various components of a plant extract from a plant tissue extract known to have a nobel product explaining how to isolate a pure extract from the crude mixed compound. (15 Marks)
QUESTION THREE (15 MARKS)
Discuss the plant propagation strategy/options that are available to a botanist. (15 Marks)
2
QUESTION FOUR (15MARKS)
From a mixed culture in one substrate explain the process of obtaining and tentatively identifying the various microbes in the mix. (15 Marks)




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