Sbt2255:Botanical Techniquies Question Paper
Sbt2255:Botanical Techniquies
Course:Bachelor Of Crop Protection
Institution: Meru University Of Science And Technology question papers
Exam Year:2013
QUESTION ONE (30 MARKS)
a) Explain the meaning of the following terms i. Totipotency (1 Mark) ii. Pellet (1 Mark) iii. Electrophonesis (1 Mark) iv. Nic (1 Mark) b) Distinguish between the following terms i. Accuracy and sensitivity ii. Magnification and resolution iii. Analytical and preparative chromatography iv. Swinging bucked and fixed angle rotors. (8 Marks) c) Explain the process of preparing an herbarium from the practical point. (6 Marks) d) Describe the process of achieving sterility. (6 Marks) e) Name any four safety apparatus in the lab. (2 Marks) f) Explain the four requirements in a PCR mixture. (4 Marks)
QUESTION TWO (20 MARKS)
Describe the two process of determining the exact sequence of bases on a DNA template showing their differences and similarities. (20 Marks)
QUESTION THREE (20 MARKS)
Describe in details the components of plant cell and tissue culture technology. (20 Marks)
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QUESTION FOUR (20 MARKS)
a) Discuss the use of chromatography in separation and analysis of extracts from plants. (10 Marks) b) Design a field experiment to test the effect of some soil amendment purpoted to increase moisture holding capacity of soil. (10 Marks)
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