Ich2106:Biochemistry I Question Paper
Ich2106:Biochemistry I
Course:Bachelor Of Science In Public Health
Institution: Meru University Of Science And Technology question papers
Exam Year:2014
QUESTION ONE – (30 MARKS)
(a) (i) Calculate the standard free energy change of the reaction catalyzed by the enzyme phospoglucontase, ?????????????? - 1 - ??h???????????? ? ?????????????? - 6 - ??h???????????? , given that starting with 20mM Glucose – 1- Phosphate and no Glucose – 6 – phosphate, the final equilibrium mixture at 25°?? and ???? 7 contains 1mM Glucose – 1- phosphate and 19mM Glucose – 6 – phosphate. (4 Marks)
(ii) Explain whether the reactions in the direction of Glucose – 6 – phosphate formation proceed with a loss or gain of free energy(?? = 8.315J/??????.??) (1 Mark)
(b) Explain why glucose is central in the metabolism of plants, animals and many micro- organisms. (4 Marks) (c) (i) Name the enzymes that make up the pyruvate dehydrogenase complex. (3 Marks) (ii) State vitamins that are important prosthetic groups to the enzymes in c(i) above. (3 Marks) (d) (i) State the three steps that give rise to high fidelity DNA synthesis. (3 Marks) (ii) State two major DNA repair mechanisms. (2 Marks)
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(e) (i) Explain the functions of mRNA, tRNA, and rRNA. (3 Marks) (ii) State any two ways by which eukaryotic mRNAs are post-transcriptionally modified. (2 Marks) (f) Explain the functions of the following hormones (i) Secretin (1 Mark) (ii) Gastrin (2 Marks) (iii) Cholecystokinin (2 Marks)
QUESTION TWO – (20 MARKS)
Describe the reactions of the Citric Acid cycle/TCA/Krebs cycle. (20 Marks)
QUESTION THREE – (20 MARKS)
(a) Derive the Michaelis-Menten equation and state its importance. (13 Marks) (b) An enzyme is discovered that catalyzes the chemical reaction; ?????????????????? ? ?????????????? A team of motivated researchers set out to study the enzyme, which they call productase. They find that the Kcat for productase is 600??-1. They carry out several experiments. When ???? = 20???? and ?????????????????? = 40???? the reaction velocity ??0???? 9.6??????-1. Calculate ???? for the substrate SUBSTRATE and determine ???????? . (7 Marks)
QUESTION FOUR – (20 MARKS)
(a) Describe the ??-oxidation of a saturated fatty acid such as the ??6 Palmitoyl-coA. (9 Marks) (b) Describe the formation of Ketone bodies from acetyl coA. (11 Marks)
QUESTION FIVE – (20 MARKS)
(a) Illustrate the excretory forms of nitrogen; ammonium ion, urea and uric acid. (6 Marks) (b) Describe the reactions for amino group catabolism in vertebrate liver. (14 Marks)
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