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Bioc 303: Integrated Laboratory Techniques 2 Question Paper

Bioc 303: Integrated Laboratory Techniques 2

Course:Bachelor Of Science

Institution: Egerton University question papers

Exam Year:2012

SECTION A: ANSWER ALL QUESTIONS

1. Define the following terms: (5 Marks)
i. Specific activity
ii. Relative centrifugal force
2. Describe the three different ways used to assess the rate of an enzyme reaction. (10 Marks)
3. Differentiate between rate zonal centrifugation and equilibrium density centrifugation techniques. (5 Marks)
4. Discuss Isoelectric Focusing. (5 Marks)
5. Explain how homogenization is achieved by osmotic alterations and ultrasonification. (4 Marks)
6. Describe analytical centrifugation. (4 Marks)
7. If a myosin is subjected to sedimentation:
(a) Give the equation that would describe its sedimentation rate (3 Marks)
(b) Explain the factors that determine its sedimentation rate (4 Marks)

SECTION B: ANSWER THREE QUESTIONS

8. With examples, describe the use of coupled enzyme reactions in enzyme assays. (10 Marks)
9. With examples, describe luminescence and manometric methods that are used in enzyme assays. (10 Marks)
10. Describe the Sodium Dodecyl Sulfate-Poly Acrylamide Gel Electrophoresis (SDS-PAGE) technique. (10 Marks)
11. Describe the identification of the N-terminal residues of a polypeptide using both the Sanger reagent and
dansyl chloride. (10 Marks)

Bioc 303: Integrated Laboratory Techniques 2 Question Paper

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