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Bmed 222: Microbial Physiology And Genetics Question Paper

Bmed 222: Microbial Physiology And Genetics

Course:Bachelor Of Science In Biomedical Science And Technology

Institution: Egerton University question papers

Exam Year:2013

SECTION A: ATTEMPT ALL QUESTIONS

1. Distinguish between the following terms: (10 Marks)
a. Transformation and trasduction
b. Resistance and virulence plasmids
c. Fermentation and aerobic respiration
d. Polycistronic and monocystronic genes
e. Archaebacteria and eubacteria
2.(a) Explain briefly the difference between G positive and G negative. Use
illustrations. (4 Marks)
(b) Why are cell walls a better pathogenic bacteria drug targets as compared to
the plasma membrane? (3 Marks)
(c) Explain the classification of prokaryotes based on pH they thrive in. (3 Marks)
3. Outline the enzymes involved in bacterial replication and state their functions. (10 Marks)
4.(a) Outline the structural organization of the lactose operon. (4 Marks)
(b) What would happen to an E. Coli bacteria in a growth media with limited glucose
but plenty of lactose sugars? (3 Marks)
(c) What would happen to E. Coli if the Lac I gene is mutated such that its
product cannot bind allolactose? (3 Marks)

SECTION B: ANSWER ANY THREE QUESTIONS

5.(a) Compare and contrast the mode of action and resistance of beta lactams and
vancomycin antibiotics. (5 Marks)
(b) Using examples compare and contrast autolysins and lysozymes. (5 Marks)
6.(a) List the major precussor metabolites that the E, Coli bacteria tap from
glycolysis for other biosynthetic purposes. (5 Marks)
(b) Account for the 38 ATP molecules that E. Coli bacteria generate from catabolism
of 1 molecule of glucose. (5 Marks)
7. What is a bacterial generation time? Briefly discuss the bacterial growth curve in
a closed system. (10 Marks)
8. Discuss in details the bacterial cell wall biosynthesis. (10 Marks)
9.(a) Giving examples, briefly describe the various nutritional types of prokaryotes. (5 Marks)
(b) Describe bacterial conjugation and indicate its medical importance. (5 Marks)

Bmed 222: Microbial Physiology And Genetics Question Paper

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