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Pmt 415:Molecular Diagnostics And Immunotechnology Question Paper

Pmt 415:Molecular Diagnostics And Immunotechnology

Course:Bachelor Of Science In Medical Biotechnology

Institution: Maseno University question papers

Exam Year:2016

MASENO UNIVERSITY

UNIVERSITY EXAMINATIONS 2016/2107

FOURTH YEAR FIRST SEMESTER EXAMINATIONS FOR THE
DEGREE OF BACHELOR OF SCIENCE IN MEDICAL BIOTECHNOLOGY
WITH INFORMATION TECHNOLOGY

MAIN CAMPUS

PMT 415: MOLECULAR DIAGNOSTICS AND IMMUNOTECHNOLOGY

Date: 1st December, 2016 Time: 12.00 - 3.00pm

INSTRUCTIONS:
.Answer ALL questions in SECTION A and TWO questions in SECTION B.

MASENO UNIVERSITY ISO 9001:2008 CERTIFIED

SECTION A:(40 MARKS): Answer all questions. Each question carries equal value

1.Outline the major steps in Polymerase Chain Reaction (5 Marks)

2.Describe how you would isolate nucleic acids for molecular diagnostic test(5 Marks)

3.Explain how you would use Fluorescent In Situ Hybridization to detect Mycobacterium tuberculosis
(5 Marks)

4.Describe any Five advantages of molecular diagnostic techniques over convectional methods of disease diagnosis (5 Marks)

5.Explain how you would use micro-array technique to detect Hepatitis B Virus (5 Marks)

6.Describe medical applications of Enzyme-Linked Immunosorbent Assay (5 Marks)

7.Describe th major steps of any Molecular assay (5 Marks)

8.Explain how you would use loop-mediated isothermal amplification(LAMP) to detect bacteria(5 Marks)

SECTION B(30 MARKS): Answer question 9 and ANY OTHER ONE question

9.Describe in detail clinical applications of DNA and RNA probes (15 Marks)

10.Describe the molecular markers useful in HIV diagnosis (15 Marks)

11.Describe PCR-based Restriction Fragment Length Polymorphism and outline its application in parasitic disease diagnosis (15 Marks)

Pmt 415:Molecular Diagnostics And Immunotechnology Question Paper

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