Pmt 313: Biochemical Techniques Ii Question Paper
Pmt 313: Biochemical Techniques Ii
Course:Bachelor Of Science In Medical Laboratory Sciences
Institution: Maseno University question papers
Exam Year:2012
MASENO UNIVERSITY
UNIVERSITY EXAMINATIONS 2012/2013
THIRD YEAR FIRST SEMESTER EXAMINATIONS FOR
THE DEGREE OF BACHELOR OF SCIENCE IN MEDICAL
BIOTECHNOLOGY/MEDICAL LABORATORY SCIENCE/
PHARMACEUTICAL SCIENCE WITH INFORMATION
TECHNOLOGY
(MAIN CAMPUS - SPECIAL EXAMS)
PMT 313: BIOCHEMICAL TECHNIQUES 11
Date: 29th July, 2013 Time: 2,00- 4.00 p.m.
MASENO UNIVERSITY ISO 9001 :2008 CERTIFIED
PMT 313 BIOCHEMICAL TECHNIQUES 11
BSc. Medical Biotechnology, BSc. Pharmaceutical Sciences and BSc. Medical
Laboratory Science
Special Examination
Section A: Short Answer Section (40 marks)
Answer ALL questions from this section
1.Give any FIVE chemical methods you can use to determine the primary structure of a protein (5 marks)
2.Explain the factors that influence protein characterization by electrophoresis (5 marks)
3.Describe the basic principles of isoelectric focusing as a method of protein separation (5 marks)
4.Explain clearly the principle of reversed-phase high performance liquid
Chromatography (5 marks).
5.Explain any THREE reasons why a biochemist would purify a protein (5 marks)
6.Describe any THREE areas where enzyme-linked immunosorbent assays are applied (5 marks)
7.Describe any THREE factors you would control for in ion-exchange
chromatography (5 marks)
8.Describe the interactive forces in centrifugation (5 marks)
Section B: Essay Section (30 marks)
Answer any TWO questions from this section
1. Describe the centrifugation methods of protein isolation (15 marks)
2. Describe the chromatographic methods of protein purification (15 marks)
3. Describe the major methods of protein characterization (15 marks)
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