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Pmt 313: Biochemical Techniques Ii Question Paper

Pmt 313: Biochemical Techniques Ii

Course:Bachelor Of Science In Medical Laboratory Sciences

Institution: Maseno University question papers

Exam Year:2012

MASENO UNIVERSITY

UNIVERSITY EXAMINATIONS 2012/2013

THIRD YEAR FIRST SEMESTER EXAMINATIONS FOR

THE DEGREE OF BACHELOR OF SCIENCE IN MEDICAL

BIOTECHNOLOGY/MEDICAL LABORATORY SCIENCE/
PHARMACEUTICAL SCIENCE WITH INFORMATION
TECHNOLOGY

(MAIN CAMPUS - SPECIAL EXAMS)

PMT 313: BIOCHEMICAL TECHNIQUES 11

Date: 29th July, 2013 Time: 2,00- 4.00 p.m.

MASENO UNIVERSITY ISO 9001 :2008 CERTIFIED

PMT 313 BIOCHEMICAL TECHNIQUES 11

BSc. Medical Biotechnology, BSc. Pharmaceutical Sciences and BSc. Medical

Laboratory Science

Special Examination

Section A: Short Answer Section (40 marks)

Answer ALL questions from this section

1.Give any FIVE chemical methods you can use to determine the primary structure of a protein (5 marks)

2.Explain the factors that influence protein characterization by electrophoresis (5 marks)

3.Describe the basic principles of isoelectric focusing as a method of protein separation (5 marks)

4.Explain clearly the principle of reversed-phase high performance liquid

Chromatography (5 marks).

5.Explain any THREE reasons why a biochemist would purify a protein (5 marks)

6.Describe any THREE areas where enzyme-linked immunosorbent assays are applied (5 marks)

7.Describe any THREE factors you would control for in ion-exchange
chromatography (5 marks)

8.Describe the interactive forces in centrifugation (5 marks)

Section B: Essay Section (30 marks)

Answer any TWO questions from this section

1. Describe the centrifugation methods of protein isolation (15 marks)

2. Describe the chromatographic methods of protein purification (15 marks)

3. Describe the major methods of protein characterization (15 marks)

Pmt 313: Biochemical Techniques Ii Question Paper

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