Pmb 448: Principles Of Genomics And Proteomics Question Paper
Pmb 448: Principles Of Genomics And Proteomics
Course:Bachelor Of Science In Medical Biotechnology
Institution: Maseno University question papers
Exam Year:2013
MASENO UNIVERSITY
UNIVERSITY EXAMINATIONS 2012/2013
FOURTH YEAR SECOND SEMESTER EXAMINATIONS
FOR THE DEGREE OF BACHELOR SCIENCE IN
MEDICAL BIOTECHNOLOGY WITH INFORMATION
TECHNOLOGY
(MAIN CAMPUS)
PMB 448: PRINCIPLES OF GENOMICS AND PROTEOMICS
Date: 5th April, 2013 Time: 8.30 -10.30am
MASENO UNIVERSITY ISO 9001:2008 CERTIFIED
PMB 448: Principles of Genomics and proteomics
Bachelor of Science in Medical Biotechnology
Section A
Instructions: answer all questions in this section.
I.Is protein chemistry same as proteomics, Explain your answer(5 marks)
2. Define the following terms (5 marks)
i) tblastx
ii) CpG islands
iii) Microsatellites
iv) Expressed sequence tags
V) Mapping reagent
3. Using a figure Where appropriate shortly describe the page electrophoresis and its application in post genomics/proteomics studies (5 Marks)
4. For analysis using Mass Spectrometry, Protein Peptide ions are usually fragmented; briefly delineate the nomenclature used to describe this peptide ion fragmentation.(5 marks)
5. Shortly describe how real genes may be distinguished from chance ORFs within a genome (5 marks)
6. What is peptide mass finger-printing (5 marks)
7. Describe Clone Contig assembly by chromosome walking (5 marks)
8. Shortly describe what the term Genome Wide Associated Studies entails
(5 marks)
PART B
Instructions: Question 9 is compulsory; additionally answer One question between Questions 10 and 11.
9. The Fourth year class in the school of genome sciences at Maseno University has been tasked with re-sequencing the bacterium Hoemophilus influenzae genome, using the shotgun approach to genome sequencing. give a detailed account of how they would go about this task. (15 MARKS)
10. MercOnyango Japunda would like to study the transcriptome of yeast cells under environmental stress in comparison to normal yeast cells, what tool will he use and how will he go about his study. (15 marks)
11. Describe how proteomic analysis may be accomplished using the MALDI-TOF Mass spectrometry. (15 marks)
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