Hbb 2115:Principles Of Biochemistry Question Paper
Hbb 2115:Principles Of Biochemistry
Course:Bachelor Of Science In Applied Biology, Genomic Science And Aquatic Science
Institution: Jomo Kenyatta University Of Agriculture And Technology question papers
Exam Year:2016
JOMO KENYATTA UNIVERSITY OF AGRICULTURE AND TECHNOLOGY
UNIVERSITY EXAMINATION FOR THE BACHELOR OF SCIENCE IN APLLIED
BIOLOGY,GENOMIC SCIENCE AND AQUATIC SCIENCE
HBB 2115:PRINCIPLES OF BIOCHEMISTRY
DATE:APRIL 2016
TIME:2 HOURS
INSTRUCTIONS:ANSWER QUESTION ONE AND ANY OTHER TWO QUESTIONS
QUESTION ONE(30 marks)
(a)Based on the monomeric units,distinguish between the three classes of carbohydrates.
(3 marks)
(b)With the aid of a Fisher Projection representation of carbohydrates,explain the
difference between aldoses and ketoses.
(3 marks)
(c)Most organic matter on the earth's surface are made up of carbohydrates.Based on
this statement,state six functions of carbohydrates.
(3 marks)
(d)Using Fisher projection representation of Glucose as an example,explain D and L
configuration of carbohydrates.
(3 marks)
(e)Differentiate between the following derivatives of sugars:
(6 marks)
i)Sugar phosphates
ii)Deoxy sugars
iii)Amino sugars
(f)Proteins play crucial roles in virtually all biological processes.Enumerate their
significance and the remarkable slope of their activity. (3 marks)
(g)State three functions of buffers. (3 marks)
(h)Differentiate between saturated and unsaturated fatty acids. (3 marks)
(i)Define the following terminology in relation to enzymes. (3 marks)
(i)Active site
(ii)Inhibitors
(j)Explain the principle of gas liquid chromatography as a biochemical technique.
(3 marks)
QUESTION TWO(20 Marks)
a)Lactose and maltose are some of the major disaccharides of physiological importance.
With the aid of Haworth projection representation,write the structural equations
illustrating the O-glycosidie linkages between the monomeric units in each. (10 marks)
b)With reference to glycogen,a major form of stored carbohydrate in animals.Explain
the O-glycosidie linkages occurring in it with the aid of structures of its monomer.
(10 marks)
QUESTION THREE(20 Marks)
Explain the biochemical principles of the following biochemical techniques: (20 Marks)
a)Column chromatography
b)Electrophoresis
c)Spectrophotometry
d)Precipitation
QUESTION FOUR (20 Marks)
a)Discuss the general structures of amino acids with the aid of a Fisher representation
highlighting each component. (8 marks)
b)Explain the classes of enzymes giving a relevant example for each class. (12 marks)
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