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Abt 334 :Tissue Culture Techniques And Practices Question Paper

Abt 334 :Tissue Culture Techniques And Practices

Course:Bachelor Of Science In Horticulture

Institution: University Of Eldoret question papers

Exam Year:2018

UNIVERSITY OF ELDORET
SCHOOL OF AGRICULTURE AND BIOTECHNOLOGY
BACHELOR OF SCIENCE IN HORTICULTURE
THIRD YEAR FIRST SEMESTER, 2017/2018 ACADEMIC YEAR
ABT 334 :TISSUE CULTURE TECHNIQUES AND PRACTICES
INSTRUCTIONS; answer all questions.
Question 1
Write short notes on the following terminologies as used in tissue culture :
a. Wide hybridization. (2 marks)
b. Explant. (2 marks)
c. Media browning. (3 marks)
d. Culture room. (3marks)
e. Tissues. (4 marks)
Question 2
A business man wishes to venture into the new business of propagating and selling high quality disease -free ornamental and fruits plantlets in Eldoret. Advice the businessman on how he should design /layout his tissue culture laboratory in order to succeed at his new business venture, giving details and the reasons why in your advice. (14marks)
Question 3
Plant tissue culture relies on the fact that many plant cells have the ability to regenerate in to a whole plant. Briefly discuss flower culture in plant tissue culture. (14 marks)
Question 4
Embryo culture is a technique that can be used to develop wide crosses in the hybrids that do not form due to pee or post fertilization barriers.
a) differentiate between embryo culture and embryo rescue. (4marks)
b) state the factors which hinder effective pre or post fertilization. (3marks)
c) Describe the types of embryo that can be cultured. ( 4marks)
d) Describe at least six applications of embryo culture. (3marks)
Question 5
Aseptic is a very important technique in tissue culture and can determine the success of plantlets under culture.
a) State at least 4 equipments and their functions found at the transfer area in a tissue culture laboratory. (2 marks)
b) As a tissue culture expert describe how one would sterilize the Explant and the media components in a tissue culture laboratory. (3marks)
c) State the advantages and disadvantages of using liquid media in plant tissue culture. (5marks)
d) State the methods that are used in vitro conservation of germplasm. (4marks)

Abt 334 :Tissue Culture Techniques And Practices Question Paper

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