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Abt 334:Tissue Culture Techniques Question Paper

Abt 334:Tissue Culture Techniques

Course:Bachelor Of Science In Biotechnology

Institution: University Of Eldoret question papers

Exam Year:2018

SAGR-BIOTECHNOLOGY DEPARTMENT
ABT 334:TISSUE CULTURE TECHNIQUES AND PRACTICES
THIRD YEAR FIRST SEMSTER 2017/2018 CAT
ANSWER ALL QUESTIONS
1.Fill in the blanks (3 mks)
a)The undifferentiated and un organized mass of cells in tissue cultures is called
a)colours
b)callus
c)callose
d) tissue
b) select the incorrectly matched pair
Protoplast-plant cell without cell wall
Cytokinin-root initiation from callus
Somatic embryogenesis-embryo produced from a vegetative cell
Anther culture- haploid plant production
2.Describe briefly the environmental factors that may influence the growth and differentiation process during culture incubation (4mks)
3. Asepsis is a very important technique in tissue culture.describe the techniques to use in the sterilization of equipments and media components (5 mks)
4)many different explants can be used for tissue culture
a) state the role of auxins in tissue culture medium(1mk)
b)which type of medium is best for protoplast cultures(1 mk)
c)discuss the factors affecting the type of culture medium to be used (5 mks)
6 somaclonal variation is a phenomenon that has been reported to occur in some plant species
Define the term somaclonal variation
What are the limitations associated with somaclonal variation
7 Germplasm is very important in agriculture for future generations. Describe ways in tissue culture can be used as means of conserving germplasm
8 Based on the group work assignment or presentation,briefly explain the culture type that you were dealing with in your group (5mks)

Abt 334:Tissue Culture Techniques Question Paper

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