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Protein And Enzymes I Question Paper

Protein And Enzymes I

Course:Bachelor Of Biochemistry

Institution: Kenyatta University question papers

Exam Year:2009

KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2009/2010
FIRST SEMESTER EXAMINATION FOR DEGREE OF BACHELOR OF SCIENCE
(BIOCHEMISTRY /BIOTECHNOLOGY)
SBC 103 N: PROTEIN AND ENZYMES I
=================================================================
DATE: MONDAY 21ST DECEMBER 2009 TIME: 2.00 P.M. – 4.00 P.M.
INSTRUCTIONS
SECTION A
Answer any TWO Quesitons. (20 marks each)

1. a) Describe the dipolar nature of a peptide bond. (3 marks)
b) What is the role of phi and psi rotational angels in protein structure.(4 marks)
c) Illustrate how a ß turn is accomplished in protein tertiary structure (3 marks)
d) What is the biological role of loops and turns in protein structure. (10 marks)

2. a) Differentiate between domains and motifs in polypeptide structure. (8 marks)

b) Describe the role of the following in the elucidation of protein structure:
i) 1-Fluoro 2, 4 dinitrobenzene (2 marks)
ii) Phenylisothiocyanate (4 marks)

c) Explain how enzymes affect biochemical reaction rates and not equilibrium.
(6 marks)

3. a) Describe a steady state enzyme catalyzed reaction. (5 marks)
b) Explain what is
i)Competitive inhibition (3 marks)
ii)Un-competitive inhibition (3 marks)
iii)Mixed inhibition (3 marks)
c) Discuss what zymogens are regarding enzyme regulation. (6 marks)

SECTION B: Answer ALL Quesitons. (10 marks each)
1. i) What is the role of auxiliary proteins in protein folding. (5 marks)
ii) The thermodynamic free energy of protein folding is inversely proportional to
the progressive attainment of its native conformation. Discuss. (5 marks)

2. Discuss the significance of Michaelis – Menten constant and maximum initial
velocity of enzyme catalysis. (10 marks)

3. Define enzyme cofactors and discuss the various groups of cofactors. (10 marks)

Protein And Enzymes I Question Paper

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