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Biochemistry And Biotechnology Question Paper

Biochemistry And Biotechnology

Course:Bachelor Of Science In Biotechnology

Institution: Kenyatta University question papers

Exam Year:2009

KENYATTA UNIVERSITY
UNIVERSITY EXAMINATIONS 2009/2010
FIRST SEMESTER EXAMINATION FOR THE DEGREE OF
BACHELOR OF SCIENCE
(BIOCHEMISTRY AND BIOTECHNOLOGY)
SBC 334: BIOCHEMISTRY OF MICROORGANISMS

DATE: Wednesday 30th December 2009 TIME: 8.00am – 10.00am

INSTRUCTIONS:

Section A: Answer Any Two Questions (20 marks each)

1. a) Name the bacteria which use lactic acid fermentation. [3marks]

b) Write the reactions of heterolactic fermentation by bacteria using
phosphoketolase pathway of glucose breakdown. [9marks]

c) State the natural habitats of lactic acid producing bacteria [4marks]

d) Explain the importance of Lactobacillus in human health. [4marks]

2. a) i) Draw the respiration chain of Paracoccus. [4marks]

ii) State the types of respiration for this bacterium. [1mark]

b) Write the reactions of hydrogen and electron transfer in the respiration
chain of Paracoccus. [11marks]

c) Indicate the sites of the formation of ATP in the above respiration
chain (a) [2marks]

d) What is the metabolic advantage of interconversion of NADH and
NADPH in Paracoccus? [2marks]

3. a) Name the bacteria growing on the methanol/formaldehyde substrates.
[2marks]

b) Write the reactions of formaldehyde assimilation via serine pathway.
[12marks]

c) State the fate of produced Acetyl-CoA and NADH in the above cycle (b).
[2marks]

d) Explain the environmental role of the methanol utilizing bacteria.
[4marks]

Section B: Answer All questions (10 marks each)

4. a) Outline the light-harvesting process of photosynthentic unit of Purple
bacteria. [4marks]

b) With the aid of diagram, show the electron and hydrogen transfer in
reaction center and respiration chain during light-harvesting and Purple
bacteria. [6marks]

5 a) Illustrate the reactions of catechol ortho-cleavage by bacteria.
[7marks]

b) State the importance of this metabolism in the biological cycles.
[3marks]

6. a) Name the non-methanogenic bacteria, which ferment biological polymers
and produce substrates for methanogens in the digestive tract of
ruminants. [2marks]

b) List the products of fermentation by non-methanogenic bacteria, which are
used as substrates for methane synthesis by methanogens. [2marks]

c) Write the reactions of methane production by the methanogens in
ruminants. [6marks

Biochemistry And Biotechnology Question Paper

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