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a) Temperature, pH value, co-factors, enzyme and co-enzymatic activity up to substrate concentration; metabolic poison/ inhibitors
b) i) Temperature - Increase in temperature increases rate of enzymatic activity up to an optimum/ low temperatures inactivates the enzymatic activity at optimum temperatures is at its best.
ii) pH - Enzymes work best at optimum pH/ extreme pH denatures enzymes. Some enzymes act best in acidic or basic medium. Optimum pH should be maintained
iii) Enzymes concentration - Increase in cooncentration increase enzymatic activity as there is more active sites to combine with substrate
iv) Co-enzymes - activates enzymes increasing rate of activity.
v) Substrate concentration - Increase in concentration increases enzymatic activity up to certain level where enzymes become a limiting factor.
vi) Inhibitors - Complete with substrate for active sites or combines permanently with active sites of enzymes. They slow down or stop the rate of reaction
johnmulu answered the question on May 1, 2017 at 07:32
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