Place the microscope on the bench with the stage facing away from you.
Turn the low power objective lens until it clicks into position.
Ensure that the diaphragm is fully open.
Look through the eye-piece with one eye; meanwhile adjust the mirror under the stage to ensure that maximum light can pass through. The circular area seen is referred to as the field of view.
Again look through the eyepiece while adjusting the mirror under the stage to ensure that sufficient light is passing through the specimen.
Use the coarse adjustment knob to bring the low power objective lens to the lowest point.
Viewing through the eye-piece, turn the coarse adjustment knob gently until the specimen comes into focus.
Use the fine adjustment knob to bring the image into sharp focus.
Make a drawing of what you observe.
For higher magnifications, turn the medium power objective lens into position and adjust the focus using the coarse adjustment knob.
For sharper images, use the fine adjustment knob.
If finer details are required, turn the high power objective lens into position; now use only the fine adjustment knob to bring the details into sharper focus.
vindori answered the question on April 17, 2018 at 13:16
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