Get premium membership and access questions with answers, video lessons as well as revision papers.
1. Ascending chromatography: As the name indicates, the chromatogram ascends. Here, the development of paper occurs due to the solvent movement or upward travel on the paper.
The solvent reservoir is at the bottom of the beaker. The paper tip with sample spots just dips into the solvent at the bottom so that spots remain well above the solvent.
2. Descending chromatography: Here, the development of paper occurs due to solvent travel downwards on the paper. The solvent reservoir is at the top. The movement of the solvent is assisted by gravity besides the capillary action. 3. Ascending- descending mode: Here solvent first travels upwards and then downwards on the paper.
4. Radial mode: Here, the solvent moves from the center (mid-point) towards the periphery of circular chromatography paper. The entire system is kept in a covered Petri dish for the development of the chromatogram.
The wick at the center of paper dips into the mobile phase in a petri dish, by which the solvent drains on to the paper and moves the sample radially to form the sample spots of different compounds as concentric rings.
5. Two-dimensional chromatography: Here the chromatogram development occurs in two directions at right angles.
In this mode, the samples are spotted to one corner of rectangular paper and allowed for first development. Then the paper is again immersed in the mobile phase at a right angle to the previous development for the second chromatogram.
Titany answered the question on September 13, 2021 at 06:41
- List four uses and applications of Paper Chromatography (Solved)
List four uses and applications of Paper Chromatography
Date posted: September 13, 2021. Answers (1)
- Discuss five classification of enzymes(Solved)
Discuss five classification of enzymes
Date posted: September 13, 2021. Answers (1)
- Describe how enzymes work(Solved)
Describe how enzymes work
Date posted: September 13, 2021. Answers (1)
- List four characteristics of enzymes(Solved)
List four characteristics of enzymes
Date posted: September 13, 2021. Answers (1)
- Draw a table to showing the recipes for common solutions (Solved)
Draw a table to showing the recipes for common solutions
Date posted: September 13, 2021. Answers (1)
- List two safety precautions when preparing solutions and reagents(Solved)
List two safety precautions when preparing solutions and reagents
Date posted: September 13, 2021. Answers (1)
- Describe the Nucleic Acids(Solved)
Describe the Nucleic Acids
Date posted: September 13, 2021. Answers (1)
- Give the structural composition of lipids(Solved)
Give the structural composition of lipids
Date posted: September 13, 2021. Answers (1)
- Give the structural composition of Proteins(Solved)
Give the structural composition of Proteins
Date posted: September 13, 2021. Answers (1)
- Give the structural composition of carbohydrates(Solved)
Give the structural composition of carbohydrates
Date posted: September 13, 2021. Answers (1)
- Define the following terms:
i. biochemical compound
ii. Chemical bond
iii. Monomer
1v. Polymer(Solved)
Define a biochemical compound
Date posted: September 13, 2021. Answers (1)
- List the characteristics of Biochemical Compounds (Solved)
List the characteristics of Biochemical Compounds
Date posted: September 13, 2021. Answers (1)
- Draw a table to show the four classes of biochemical compounds(Solved)
Draw a table to show the four classes of biochemical compounds
Date posted: September 13, 2021. Answers (1)
- Give the methods involved in cell separation and isolation(Solved)
Give the methods involved in cell separation and isolation
Date posted: September 13, 2021. Answers (1)
- What is facilitated transfusion in plants?(Solved)
What is facilitated transfusion in plants?
Date posted: September 13, 2021. Answers (1)
- What is Cell Metabolism?(Solved)
What is Cell Metabolism?
Date posted: September 10, 2021. Answers (1)
- Differentiate between a plant and an animal cell(Solved)
Differentiate between a plant and an animal cell
Date posted: September 10, 2021. Answers (1)
- Functions of the cell wall (Solved)
Functions of the cell wall
Date posted: September 10, 2021. Answers (1)
- Types of Cells in the Human Body (Solved)
Types of Cells in the Human Body
Date posted: September 10, 2021. Answers (1)
- Give the methods used for sterilization in the lab(Solved)
Give the methods used for sterilization in the lab
Date posted: September 10, 2021. Answers (1)