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BMB 421 – Gene expression systems and sequencing - Microarrays Notes

Institution: Masinde Muliro University of Science and Technology

Course: BSc Medical Biotechnology

Content Category: Class Notes

Posted By: mikeGreen

Document Type: DOCX

Number of Pages: 10

Price: KES 120
 
    

Views: 575     Downloads: 0

Summary

Details of different types of microarrays (a laboratory tool used to detect the expression of thousands of cellular molecules at the same time) is discussed. The paper includes; RNA, DNA, Peptide, Cellular and Protein Microarrays.
These notes covers up to, principles, applications, Technology involved and their applications in the field.
The work has relevant references and reliable for further studies.

DNA microarray
A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. Each DNA spot contains Pico moles (10-12 moles) of a specific DNA sequence, known as probes (or reporters or oligos). These can be a short section of a gene or other DNA element that are used to hybridize a cDNA or cRNA (also called anti-sense RNA) sample (called target) under high-stringency conditions. Probe-target hybridization is usually detected and quantified by detection of fluorophore-, silver-, or chemiluminescence-labeled targets to determine relative abundance of nucleic acid sequences in the target.


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